DNA
Part:BBa_I751401:Design
Designed by: Kenichiro Iwasaki Group: iGEM07_Tokyo_Tech (2007-10-25)
BioBrick to Tokyo Standard adapter, for XbaI, reverse
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 6
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
1. Cut Biobrick part with XbaI.
2. Incorporate adapter oligo. (don't forget to anneal with fwd, and to make kination)
3. Now you can cut out this part by SalI or BamHI.
This makes Biobrick scar-like sequence, "tctagt" in the left and "actaga" in the right. Once oligo is ligated, you can't cut with XbaI anymore.